P Egfr Antibody Cell Signaling

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P egfr antibody cell signaling.

1999 trends pharmacol sci 20 408 12. Mutation of either of these serines results in upregulated egfr tyrosine autophosphorylation 10. Egfr carboxy terminal residues ser1046 and ser1047 are phosphorylated by cam kinase ii. Zero bias scores article reviews protocol conditions and more.

Quantitation of activated signaling molecules. Rabbit recombinant monoclonal egfr phospho y1068 antibody ep774y. P egfr antibody 12a3 is a mouse monoclonal igg 1 provided at 50 µg 0 5 ml. Antibodies to beta tubulin or beta actin were used as loading controls and for semi quantitation.

A431 whole cell lysate 100ng ml egf for 1 minute lane 3. Akt pser473 antibody arg51558 a. Top 3 egf signaling must haves. Phosphorylation of egfr at specific serine and threonine residues attenuates egfr kinase activity.

A431 whole cell lysate 100ng ml egf for 2 5 minutes lane 4. Egfr antibody a 10 is available as both the non conjugated anti egfr antibody form as well as multiple conjugated forms of anti egfr antibody. 1999 curr opin cell biol 11 184 9. Mir 146a treatment decreased whereas its inhibitor increased protein levels of p egfr p erk1 2 p p38 and not significantly p akt.

Hela egf 5 mins c. Anti egfr phospho y1068 antibody ab5644 at 1 200 dilution lane 1. Egfr carboxy terminal residues ser1046 and ser1047 are phosphorylated by cam kinase ii. Egfr antibody a 10 is a high quality monoclonal egfr antibody also designated her1 antibody or erbb1 antibody suitable for the detection of the egfr protein of mouse rat and human origin.

Non cross reactive with the non phosphorylated egfr nor with unrelated tyrosine phosphorylated proteins. Validated in wb ihc flow cyt dot icc if and tested in mouse human. Monoclonal antibody is produced by immunizing animals with a fusion protein containing the cytoplasmic domain of human egf receptor. A431 whole cell lysate 100ng ml egf for 5 minutes.

Hela egf 10 mins akt also known as protein kinase b pkb is a serine threonine specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism apoptosis cell proliferation transcription and cell migration. Recommended for detection of tyr 845 phosphorylated egfr of mouse rat and human origin by wb and ip. 1999 curr opin cell biol 11 184 9. Epidermal growth factor receptor proto oncogene c erbb 1 receptor tyrosine protein kinase erbb 1 egfr human.

Raised against an egfr phosphopeptide of human origin. Independently reviewed in 14. A431 whole cell lysate not treated lane 2. 1993 cell 73.